Application of RAPD-PCR and Phylogenetic Analysis for Accurate ‎Characterization of ‎Salmonella‎‎‎ spp. Isolated from Chicken and Their Feed ‎and Drinking Water in Comparison ‎with API 20E, Vitek 2, and Serotyping


 The aim of this study was ‎the‎ discrimination Salmonella‎‎ isolated from chicken and their feed ‎and drinking water for the epidemiological control salmonellosis. Totally, 289 samples, ‎including 217 cloaca swabs, 46 water, 26 samples were collected five ‎different farms in Karbala governorate, Iraq. Conventional bacteriology tests, API 20E, Vitek 2, serology used bacterial identification. Random amplified polymorphic ‎DNA (RAPD)-polymerase chain reaction (PCR) applied to analyze genetic relationships ‎among isolates. isolation rate spp. 21.1% (61/289). While ‎water constituted highest (30.4%), a 21.7% reported ‎swabs, with no isolate at all feed. 2 able identify some isolates ‎serotype level, such as S. Enteritidis, Paratyphi B, C. However, ‎were diagnosed enterica by subsp. arizonae through serology. ‎Analyzing RAPD-PCR assay showed presence genetically different ‎Salmonella‎‎ Dendrograms created GelJ software successfully delineated ‎relationships. Therefore, can be surrogate tool fast, reliable, ‎accurate detection surveys when compared other ‎biochemical-based identification methods.